Downloaded 43 times
Uploaded byInsideScientific
Simple Tips to Significantly Improve Rodent Surgical Outcomes
The document outlines six essential tips to improve surgical outcomes in rodents, emphasizing acclimation, aseptic techniques, temperature regulation, hydration, oxygen supplementation, and non-pharmaceutical pain control methods. It highlights the importance of a sterile environment and appropriate care to minimize physiological stress and enhance recovery in laboratory settings. The author, Marcel Perret-Gentil, provides practical advice for veterinary practices at the University of Texas at San Antonio.
More Related Content
Similar to Simple Tips to Significantly Improve Rodent Surgical Outcomes
Simple Tips to Significantly Improve Rodent Surgical Outcomes
- 1. Copyright 2023. AllRights Reserved. Contact Presenter for Permission. University Veterinarian & Director Laboratory Animal Resources Center The University of Texas at San Antonio (UTSA) Marcel Perret-Gentil, DVM, MS Simple Tips to Significantly Improve Rodent Surgical Outcomes
- 2. 6 Simple Tips thatwill Significantly Improve Rodent Surgical Outcomes University Veterinarian & Director The University of Texas at San Antonio marcel.perret@utsa.edu https://research.utsa.edu/complia nce/larc/training.html Marcel Perret-Gentil President PLAVS (Perret-Gentil Lab Animal Veterinary Services) mperret1@gmail.com www.plavs.net
- 3. 3 Ms. Rita is85 An exemplary nurse Just a Little bit out of practice
- 4. The great tips 1.Acclimation 2. Aseptic technique – the ‘solo surgeon syndrome’ 3. Temperature regulation 4. Hydration 5. Oxygen supplementation 6. Non-pharmaceutical methods of pain control
- 5. Tip 1 –Acclimation
- 6.
- 7.
- 8. Tip 2 –Aseptic Surgery?
- 9. Survival alone nota valid criterion for success Success should be based on absence of altered physiological function, immune responses and behavioral changes (Cunliffe-Beamer, 1993) 9
- 10. Aseptic technique increasessuccess of ovarian transplants in mice & speeds return to post-op normal (Cunliffe-Beamer 1972-73; Cunliffe-Beamer 1990) Contamination activates macrophages (Bancroft, Schreiber et al. 1989), and leads to changes in cytokines & B cells levels (Abbas, Lichtman et al. 1991) SUBCLINICAL infections induce physiological changes (Committee on Infectious Diseases of Laboratory Rats and Mice 1992) Although NO CLINICAL SIGNS observed, experimentally inoculated rats (108 S. aureus or P. aeruginosa) had significant alterations in plasma fibrinogen, serum glucose, total white blood cell counts, and wound histology scores (Bradfield, John et al. 1992) 10
- 11. Summary • Delays topost-op normality • Physiological changes • Alterations in immunity • Affects data yield Poor rodent aseptic surgery results in: 11
- 12. A few easytips on aseptic technique https://research.utsa.edu/compliance/larc/t raining.html for many easy tips 12
- 13. Surgeon Prep Impermeable armsleeves Misted with disinfectant 13
- 14. Crowded surgical areasetup Correct setup A bit crowded Suture touching non- sterile area 👿 14 Gauze touching non- sterile area
- 15. Easy Table &Animal Draping Technique without Sterile Gloves 15
- 16. Post-op recovery cageis dirty 👿
- 17. Post-op recovery cageis clean, to minimize post-op infections 😇
- 18. Rodent Surgical Draping The5 properties of the ideal rodent draping material… 1. Sterile 2. Adheres to skin 3. Impermeable 4. Transparent 5. Traps body heat… 18 Bonus: Cheap!
- 19. Draping the Animal ✕ 1.Sterile 2. Adheres to skin 3. Impermeable 4. Transparent 5. Traps body heat… 19 Cheap! 🙁
- 20. The Solo Surgeon Oneperson does everything What happens if non-sterile items such as the anesthesia dials, stereotaxic apparatus knobs, light handles and microscope dials have to be manipulated during surgery? The next slides provide practical solutions to the “Solo Surgeon” problem 20
- 21. Touching non- sterile parts withsterile gloves 👿 👿 👿
- 22. Press’n Seal oraluminum foil 😇 😇 😇
- 23.
- 24.
- 25.
- 26. Need to usea timer and you are the solo surgeon?
- 27. When need towrite notes and you are the solo surgeon
- 28. Tip 3 –Temperature Regulation
- 29. Anesthetics & Hypothermia •Anesthetics depress thermoregulation • Vasodilation makes it worse • Prolongs recovery • Leads to hypoventilation • ¯ platelet function • risk of infection • Bradycardia, fatal arrhythmias, myocardial infarction • blood viscosity = ¯ gas exchange • ↑ mortality • Affects data 29
- 30. Good Veterinary Care Involves Avoidanceof Hypothermia
- 31. Insulate with Press’nSeal 31 Include tail
- 32.
- 33.
- 34. Tip 4 -Hydration 34
- 35. Hydration Make it ahabit of administering WARMED fluids before surgery – Lactated Ringer’s solution or onrmal saline • Mice 0.5-1 ml SC/IP • Rats 5-10 ml SC/IP 😇 35 10-20 ml/kg
- 36. Tip 5 –Oxygen Supplementation 36
- 37. Injectable Anesthesia = Hypoxia= O2 saturation in 80s & 70s (dose dependent)* Not consistent with acceptable veterinary practice Malpractice? UTSA requires O2 supplementation 37 *Blevins et al, 2021
- 38. O2 Supplementation O2 supplementation= ↑↑↑ Survival* At UTSA - vaporizer system with anesthesia turned off to supplement O2 (0.5 L/min) via facemask when using injectable anesthetics May use O2 concentrators 38 *Blevins et al, 2021
- 39. Tip 6 –Non-Pharmacological Methods of Pain Control 39
- 40. Treating the Mind andOther Parts Simkin & O’hara, 2002; Chia et al, 2017
- 42. Fear & Anxiety EnhancePain Response Providing a stress-free environment should be considered in the pain management program Linton 2000; Morley et al. 1999; Munro et al. 2007; Panksepp 1980; Perkins and Kehlet 2000; Ploghaus et al. 2001
- 43. Minimizing Environmental Stress 43 Minimizesstress-induced hyperalgesia* *Mogil, 2017
- 44. Tasty & EnergyDense Supplementation Bacon Softies bio-Serv.com DietGel clearh2o.com 44
- 45. Social Housing Single housing– The post op norm, however… • Rats spinal cord injury – 20% less chance of survival when housed individually • Telemetric implant surgery, female mice - When housed socially needed less time to fully recover (Van Loo et al., 2007) 45
- 46. Soon!! Overview of Refinementsin Rodent Surgical Practices (JAALAS) 46