What is aProbability Distribution?
• A probability distribution tells us how the probabilities of different
outcomes are spread out for a random variable.
• In simple words:
If you have an experiment that can give different results, a
probability distribution tells you how likely each result is.
Examples in biology/parasitology:
• Number of infected individuals in a sample
• No. of Parasites in a host
• No. of eggs per gram of Faeces.
• Success/Failure of a diagnostic test.
3.
• Different typesof data follow different patterns.
If we know the pattern, we can choose the correct:
• Statistical test
• Method of analysis
• Interpretation of results
• Without this, research can go wrong because wrong distribution →
wrong test wrong conclusion
→ .
• Example:
If parasite egg counts follow Poisson, but you analyze them as Normal,
your conclusions become false.
4.
Binomial
Distributio
n
• When toUse It?
• Use it when:
• Only two outcomes exist Yes/No, Positive/Negative
→
• Number of trials (n) is fixed
• Probability of success (p) is constant
• Trials are independent
• Simple Parasitology Example
• A child is either:
• Infected (Success)
• Not infected (Failure)
• If you test 100 children for Ascaris, the pattern of infected/not
infected follows a binomial distribution.
• Why is it important for researchers?
• It helps answer:
• What is the probability that 30 out of 100 children are infected?
• Is the infection rate higher than expected?
• Is a diagnostic test performing correctly?
• You cannot correctly calculate prevalence or compare infection rates
without using binomial principles.
6.
Binomial
Distributio
n (n =10, p
= 0.5)
• Explanation of Parameters
• n = number of trials
Here, n = 10 means we perform the experiment
→ 10 times
(e.g., testing 10 children).
• p = probability of success
p = 0.5 means the chance of success in each trial is
→ 50%
(e.g., chance a child is infected = 0.5).
• Meaning of the Diagram
• The bars show the probability of getting
0, 1, 2, …, 10 successes out of 10 trials.
• The highest bar is around 5 successes, because when p = 0.5,
getting half successes is most likely.
• The shape is symmetric when p = 0.5.
• Simple Biological Example
• If infection chance is 50%, the diagram shows the probability of
having:
• 0 infected children
• 1 infected child
• 2 infected children
…
• up to 10 infected children
• out of 10 tested.
7.
Poisson
Distributio
n
• When toUse It?
• Use Poisson when:
• You are counting rare, random events
• They occur independently
• They occur at a constant average rate (λ)
• Simple Parasitology Example
• Hookworm eggs per gram of stool:
• Some children have 0
• Some have 1 or 2
• Rarely someone may have 5 or more
• This fits a Poisson distribution.
• Why is it important for researchers?
• It helps you decide:
• Is the number of eggs increasing normally or due to an
outbreak?
• Are infections randomly spread or clustered?
• Is the transmission stable?
• You cannot correctly analyze egg counts, case counts, or rare
parasitic events without Poisson distribution.
9.
Poisson
Distributio
n (λ =3)
• Explanation of λ (Lambda)
• λ (lambda) = average rate of occurrence
Here, λ = 3 means
→ the average number of events is 3
(e.g., on average 3 hookworm eggs per gram of stool).
• Meaning of the Diagram
• The bars show the probability of observing
0, 1, 2, 3, 4, … eggs per gram.
• The highest probability is at k = 3, because that is the average.
• The distribution is skewed, not symmetric.
• Simple Biological Example
• If the average egg count is 3 eggs/g, the diagram shows the chance of
finding:
• 0 eggs
• 1 egg
• 2 eggs
• 3 eggs
• up to 14 eggs
• in a sample.
Because egg counts are random and rare, Poisson fits this well.
10.
Normal
Distributio
n
• When toUse It?
• Use it for continuous biological measurements that show a bell-shaped
curve.
• Simple Biological Example
• Hemoglobin (Hb) levels of 300 children:
• Most children have Hb near the mean (e.g., 11 g/dL)
• Few have very high or very low values
• This typically forms a normal distribution.
• Why is it important for researchers?
• Normal distribution is the foundation of:
• Mean, SD
• t-test
• z-test
• Confidence intervals
• Regression analysis
• Without understanding normal distribution, you cannot correctly
compare biological measurements.
12.
Normal
Distributio
n (μ =0, σ
= 1)
• Explanation of Parameters
• μ (mu) = mean
Here, μ = 0 means the average value is 0.
→
• σ (sigma) = standard deviation
σ = 1 means values typically lie within
→
±1 of the mean for 68% of the cases.
• Meaning of the Diagram
• A smooth bell-shaped curve.
• Most data values are near the middle (mean).
• Fewer values occur near the extremes (tails).
• Symmetrical on both sides.
• Simple Biological Example
• If Hb levels or heights follow a normal distribution:
• Most children have Hb near the average
• Very few have extremely low or high Hb
• The curve explains how values are spread
13.
Summary of Notation(Very Simple
Table)
Symbol Meaning Example
n Number of trials Children tested (e.g., n = 10)
p Probability of success Infection chance (e.g., p = 0.4)
λ (lambda) Average rate of rare events Average egg count = 3
μ (mu) Mean of continuous data Average Hb level
σ (sigma) Standard deviation Spread of Hb levels
14.
A Simple Tableto Remember
If data is… Distribution Examples
Two outcomes only Binomial Infected / Not infected
Rare events counted Poisson
Egg counts, number of
cases
Continuous & natural Normal
Hb levels, heights,
weights
15.
Why You
Cannot Do
Research
Without
Probability
Distributio
ns
•They tell you which statistical test to
use.
• They help you avoid wrong
conclusions.
• They explain how nature behaves
(infection patterns, measurements,
rare events).
• They guide sampling, study design,
and interpretation.
• Distributions are like maps they
→
guide a researcher toward the correct
statistical road.