Extraction, isolation,characterization and uses of curcumin

Extraction, isolation,characterization and uses of curcumin

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  EXTRACTION,ISOLATION,CHARACTERI ZATION AND USESOF CURCUMIN PRESENT BY:- ARSHAD HUSSAIN
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  INTRODUCTION • CURCUMIN ISAN ORANGE–YELLOW CRYSTALLINE POWDER ESSENTIALLY INSOLUBLE IN WATER. • CURCUMIN IS YELLOW IN COLOR SHADE AND IS MOST PRECIOUS CONSTITUENT OF TURMERIC. • CURCUMIN IS ONE OF THE THREE CURCUMINOIDS THAT APPEAR IN TURMERIC, THE OTHER TWO BEING DESMETHOXYCURCUMIN AND BIS- DESMETHOXYCURCUMIN.
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  EXTRACTION OF CURCUMIN •CURCUMIN IS EXTRACTED FROM TURMERIC WHICH IS DERIVED FROM RHIZOME OF THE PLANT CURCUMA LONGA. • THE EXTRACTION PROCESS IS CARRIED OUT IN TWO STEPS SELECTIVE REMOVAL OF THE TURMERIC OIL AND EXTRACTION OF PIGMENT 1. RHIZOME IS DE-OILED BY HEXANE, WHICH HAS POOR SELECTIVITY OF COLOURING MATTER 2. THE RESIDUE IS THEN FEED FROM THE SOLVENT AND EXHAUSTIVELY EXTRACTED WITH ANOTHER SOLVENT LIKE METHANOL TO RECOVER CURCUMIN.AFTER REMOVING THE SOLVENT, THE CONCENTRATED EXTRACT DISSOLVED IN ALKALI,FILTERED&ACIDIFIED WITH ACID TO PRECIPITATE TO PIGMENT.
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  ISOLATION OF CURCUMIN •CURCUMIN IS INSOLUBLE IN WATER; AN ORGANIC SOLVENT HAS BEEN USED FOR ITS ISOLATION. • DEVELOPED A TECHNIQUE FOR ISOLATING CURCUMIN FROM GROUND TURMERIC. • THEY MAGNETICALLY STIRRED THE GROUND TURMERIC IN DICHLOROMETHANE AND HEATED AT REFLUX FOR 1H • THE MIXTURE WAS SUCTION-FILTERED, AND THE FILTRATE WAS CONCENTRATED IN A HOT-WATER BATH MAINTAINING AT 50°C. • THE REDDISH-YELLOW OILRESIDUE WAS TRITURATED WITH HEXANE AND THE RESULTING SOLID WAS COLLECTED BY SUCTION FILTRATION.
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  CHARACTERIZATION OF CURCUMIN •THE FABRICATED CURCUMIN IS ANALYZED WITH HIGH PER-FORMANCE LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY (HPLC/MS) OF AGILENT 1260 SERIES SINGLE QUADRUPOLE LC/MS SYSTEMS (AGILENT TECHNOLOGIES, USA. • STOCK SOLUTIONS OF CURCUMIN (200 ΜG/ML) WAS PREPARED BY DISSOLVING ACCURATELY WEIGHED 10 MG OF CURCUMIN IN METHANOL USING 50 ML VOLUMETRIC FLASK. • THIS SOLUTION WAS FURTHER DILUTED WITH METHANOL TO OBTAIN STANDARD SOLUTIONS IN THE CONCENTRATION RANGE OF 1–20 ΜG/ML. • THE SEPARATION WAS CARRIED OUT IN A ZORBAX ECLIPSE. XDB C18 COLUMN (4.6 × 150 MM, 5Μ M) WITH A C18 GUARD COLUMN MAINTAINED AT 24 °C.
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  • THE ELUTIONWAS PERFORMED AT FLOW RATE OF 0.5 ML/MIN WITH A GRADIENT MOBILE PHASE FROM 10 TO 30 % ACETONITRILE IN WATER IN 5 MIN, FOLLOWED BY AN INCREASE FROM 30 TO 70 % ACETONITRILE IN ANOTHER 20 MIN. • THE INJEC-TION VOLUME WAS OF 5 ΜL. THE DAD ACQUISITION WAVE- LENGTH FOR DEMETHOXYCURCUMIN, BISDEMETHOXYCURCUMINAND CURCUMIN DETECTION WAS SET AT 425 NM. • THE MASS DETECTOR USED A MM-ES MULTIMODE SOURCE. • OPENLAB SOFTWARE (AGILENT TECHNOLOGIES) WAS EMPLOYED TO CONTROL AND COLLECT SAMPLE. • THE ION PEAKS AT M/Z 339.0, 309.1, AND 369.1 ([M + H]+) WERE DETECTED FOR DEMETHOXYCURCUMIN, BISDEMETHOXYCURCUMIN AND CURCUMIN.
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  USES OF CURCUMIN •TURMERIC HAS A LONG HISTORY OF MEDICINAL USES, AND WAS ONCE KNOWN AS CURCUMA LONGA. • IT IS A FLAVOURING SPICE FROM A FLOWERING ROOT PLANT AND BELONGS TO THE GINGER FAMILY. • IT’S ORIGIN IS PRIMARILY INDIA AND ALSO OTHER COUNTRIES IN SOUTHEAST ASIA AND ASIDE FROM ITS COOKING USE AND BEING USED AS A SPICE FOR CENTURIES. • IT IS ALSO POPULAR FOR ITS ANTI-INFLAMMATORY AND ANTIOXIDANT PROPERTIES.
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  • 14 PROVENBENEFITS OF TURMERIC AND CURCUMIN 1. FIGHTS INFLAMMATION 2. HELPS WITH HAY FEVER 3. HELPS WITH DEPRESSION 4. COULD HELP EASE OSTEOARTHRITIS PAIN 5. DECREASES HIGH CHOLESTEROL 6. MAY PREVENT CANCERS 7. REDUCES ITCHING
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  8. MAY HELPDIABETES 9. REDUCES THE RISK OF HEART DISEASE 10. HELPS WITH THE BUILDUP OF FAT IN THE LIVER 11. CURES HANGOVERS 12. HELPS REDUCE THE RISK OF PREMENSTRUAL SYNDROME 13. HELPS TREAT INFLAMMATORY SKIN CONDITIONS 14. MAY HELP TREAT ALZHEIMER’S DISEASE
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  THANK YOU